The influence of purification of carp collagen used in a novel composite graft with sandwich construction of the wall on its biological properties and graft patency rates

Title:

The influence of purification of carp collagen used in a novel composite graft with sandwich construction of the wall on its biological properties and graft patency rates

Creator:

Mitas, P. , Grus, T. , Lambert, L., Mlcek, M. , Chlup, H. , Honsova, E. , Dohnalova, M. , Suchy, T., Burgetova, A., Lindner, J. , and Spacek, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4cf8513a-9d94-409d-924c-96ee37f5ee12
uuid:4cf8513a-9d94-409d-924c-96ee37f5ee12
doi:10.33549/physiolres.934117

Subject:

vascular graft, collagen, crosslinking, extrusion, composite tube, patency, and sheep

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

We compared graft outcome between two types of a novel composite three-layer carp-collagen-coated vascular graft in low-flow conditions in a sheep model. Collagen in group A underwent more cycles of purification than in group B in order to increase the ratio between collagen and residual fat. The grafts were implanted end-to-side in both carotid arteries in sheep (14 grafts in 7 sheep in group A, 18 grafts in 9 sheep in group B) and artificially stenosed on the right side. The flow in the grafts in group A decreased from 297±118 ml/min to 158±159 ml/min (p=0.041) after placement of the artificial stenosis in group A, and from 330±164ml/min to 97±29 ml/min (p=0.0052) in group B (p=0.27 between the groups). From the five surviving animals in group A, both grafts occluded in one animal 3 and 14 days after implantation. In group B, from the six surviving animals, only one graft on the left side remained patent (p=0.0017). Histology showed degradation of the intimal layer in the center with endothelization from the periphery in group A and formation of thick fibrous intimal layer in group B. We conclude that the ratio between collagen and lipid content in the novel three-layer graft plays a critical role in its patency and structural changes in vivo.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

603-610

Source:

Physiological research | 2019 Volume:68 | Number:4

Harvested from:

CDK

Metadata only:

false