PsbY is required for prevention of photodamage to photosystem II in a PsbM-lacking mutant of Synechocystis sp. PCC 6803

Title:

PsbY is required for prevention of photodamage to photosystem II in a PsbM-lacking mutant of Synechocystis sp. PCC 6803

Creator:

Biswas, S. and Eaton-Rye, Julian J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4992c93c-3065-4f59-94c7-0e427ebcbdcf
uuid:4992c93c-3065-4f59-94c7-0e427ebcbdcf
issn:0300-3604
doi:10.1007/s11099-018-0788-6

Subject:

hydrogenuhličitan sodný, cytochromy, ochrana před ultrafialovým zářením, bicarbonate, cytochromes, photoprotection, assembly, chlorophyll fluorescence, cytochrome b559, repair, 2, and 581

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

The PsbM (3.9 kDa) and PsbY (4.2 kDa) proteins are membrane-spanning, single-helix, subunits associated with the chlorophyll-binding CP47 pre-complex of photosystem II (PSII). Removal of PsbM resulted in accumulation of PSII pre-assembly complexes and impaired electron transfer between the primary (QA) and secondary (QB) plastoquinone electron acceptors of PSII indicating that the QB-binding site and bicarbonate binding to the non-heme iron were altered in this strain. Removal of PsbY alone had only a minor impact on PSII activity but deleting PsbY in the PsbM background led to additional modification of the acceptor side resulting in PsbM:PsbY cells being susceptible to photodamage and this required protein synthesis for recovery. Addition of bicarbonate was able to compensate for the light-induced damage in PsbM:PsbY cells potentially re-occupying the modified bicarbonate-binding site in the PsbM:PsbY strain and complementation of PsbM:PsbY cells with the psbY gene restored the PsbM phenotype., S. Biswas, J. J. Eaton-Rye., and Obsahuje bibliografické odkazy

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

200-209

Source:

Photosynthetica | 2018 Volume:56 | Number:1

Harvested from:

CDK

Metadata only:

false