Valproic acid decreases the nuclear localization of MDT-28, the nematode orthologue of MED28

Title:

Valproic acid decreases the nuclear localization of MDT-28, the nematode orthologue of MED28

Creator:

Kostrouchová, M., Kostrouchová, V., Yilma, P., Benda, A., and Mandys, V.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:46d98fec-fc94-4d8c-8594-9364e178dc3d
uuid:46d98fec-fc94-4d8c-8594-9364e178dc3d

Subject:

mediator complex, valproic acid, nuclear localization, MDT-28, and MED28

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Mediator is a multiprotein complex that connects regulation mediated by transcription factors with RNA polymerase II transcriptional machinery and integrates signals from the cell regulatory cascades with gene expression. One of the Mediator subunits, Mediator complex subunit 28 (MED28), has a dual nuclear and cytoplasmic localization and function. In the nucleus, MED28 functions as part of Mediator and in the cytoplasm, it interacts with cytoskeletal proteins and is part of the regulatory cascades including that of Grb2. MED28 thus has the potential to bring cytoplasmic regulatory interactions towards the centre of gene expression regulation. In this study, we identified MDT-28, the nematode orthologue of MED28, as a likely target of lysine acetylation using bioinformatic prediction of post-translational modifications. Lysine acetylation was experimentally confirmed using anti-acetyl lysine antibody on immunoprecipitated GFP::MDT-28 ex-pressed in synchronized C. elegans. Valproic acid (VPA), a known inhibitor of lysine deacetylases, enhanced the lysine acetylation of GFP::MDT-28. At the subcellular level, VPA decreased the nuclear localization of GFP::MDT-28 detected by fluorescence-lifetime imaging microscopy (FLIM). This indicates that the nuclear pool of MDT-28 is regulated by a mechanism sensitive to VPA and provides an indirect support for a variable relative proportion of MED28 orthologues with other Mediator subunits. and Corresponding author: Markéta Kostrouchová

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

[1]-9

Source:

Folia biologica | 2018 Volume:64 | Number:1

Harvested from:

CDK

Metadata only:

false