A versatile chamber for simultaneous measurements of oxygen exchange and fluorescence in filamentous and thallous algae as well as higher plants

Title:

A versatile chamber for simultaneous measurements of oxygen exchange and fluorescence in filamentous and thallous algae as well as higher plants

Creator:

Küpper, H., Šetlík, I., and Hlásek, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:40266686-83df-47c3-b5b9-d89aa43f58e8
uuid:40266686-83df-47c3-b5b9-d89aa43f58e8
issn:0300-3604
doi:10.1007/S11099-005-0016-z

Subject:

Antithamnion, chlorophyll fluorescence induction, Ectocarpus, gas exchange, photosynthetic oxygen release, photosynthesis, and submerged aquatic plants

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

A new chamber was developed for a simultaneous measurement of fluorescence kinetics and oxygen exchange in filamentous and thallous algae as well as in small leaves of water plants. Algal filaments or thalli are kept by a stainless grid close to the bottom window of the chamber in the sample compartment. The grid separates the object from the electrode compartment with the oxygen electrode at the top. This compartment accommodates, in addition, a magnetic stirrer that provides efficient circulation of the medium between the sample and the electrode. This magnetic bar spins on a fixed axis and is driven by an electronically commutated magnetic field produced by four coils which are arranged around the chamber. This design yields a very favourable signal to noise ratio in the oxygen electrode records. Consequently, measurements can be performed even of algae with very low photosynthetic rates such as marine low-light red algae or algae under severe stress. For irradiation of the samples and for fluorescence measurements a fibre optic light guide is used facing the window of the chamber. The four branches of a commercially available light guide serve the following purposes: collection of sample fluorescence and supply of measuring, actinic, and saturating light, respectively. and H. Küpper, I. Šetlík, M. Hlásek.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

579-583

Source:

Photosynthetica | 2004 Volume:42 | Number:4

Harvested from:

CDK

Metadata only:

false