Conplastic strains for identification of retrograde effects of mitochondrial dna variation on cardiometabolic traits in the spontaneously hypertensive rat

Title:

Conplastic strains for identification of retrograde effects of mitochondrial dna variation on cardiometabolic traits in the spontaneously hypertensive rat

Creator:

Pravenec, Michal, Šilhavý, Jan, Mlejnek, Petr, Šimáková, Miroslava, Mráček, Tomáš, Pecinová, Alena, Tauchmannová, Kateřina, Hütl, Martina, Malínská, Hana, Kazdová, Ludmila, Neckář, Jan, Kolář, František, Žurmanová, Jitka, Novotný, Jiří, and Houštěk, Josef

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3da00b91-4baf-4851-80e0-e420c6366803
uuid:3da00b91-4baf-4851-80e0-e420c6366803
doi:10.33549/physiolres.934740

Subject:

mitochondrial DNA, conplastic strains, spontaneously hypertensive rat, glucose and lipid metabolism, and cardiac traits

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Mitochondrial retrograde signaling is a pathway of communication from mitochondria to the nucleus. Recently, natural mitochondrial genome (mtDNA) polymorphisms (haplogroups) received increasing attention in the pathophysiology of human common diseases. However, retrograde effects of mtDNA variants on such traits are difficult to study in humans. The conplastic strains represent key animal models to elucidate regulatory roles of mtDNA haplogroups on defined nuclear genome background. To analyze the relationship between mtDNA variants and cardiometabolic traits, we derived a set of rat conplastic strains (SHR-mtBN, SHR-mtF344 and SHR-mtLEW), harboring all major mtDNA haplotypes present in common inbred strains on the nuclear background of the spontaneously hypertensive rat (SHR). The BN, F344 and LEW mtDNA differ from the SHR in multiple amino acid substitutions in protein coding genes and also in variants of tRNA and rRNA genes. Different mtDNA haplotypes were found to predispose to various sets of cardiometabolic phenotypes which provided evidence for significant retrograde effects of mtDNA in the SHR. In the future, these animals could be used to decipher individual biochemical components involved in the retrograde signaling.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

S471-S481

Source:

Physiological research | 2021 Volume:70 | Number:Supplement 4

Harvested from:

CDK

Metadata only:

false