Histochemical demonstration of five enzymes' activities in Macrogyrodactylus clarii (Monogenea: Gyrodactylidae) from the catfish Clarias gariepinus

Title:

Histochemical demonstration of five enzymes' activities in Macrogyrodactylus clarii (Monogenea: Gyrodactylidae) from the catfish Clarias gariepinus

Creator:

Arafa, Safaa Zaky, Abd El-Hady, El-Sayed Kasem, and El-Abbassy, Samir Ahmed

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3b342faa-3aea-49b5-9653-11967ce9ee6d
uuid:3b342faa-3aea-49b5-9653-11967ce9ee6d
doi:10.14411/fp.2012.007

Subject:

Macrogyrodactylus clarii, Clarias gariepinus, histochemistry, alkaline phosphatase, acid phosphatase, adenosine triphosphatase, 5'-nucleotidase, and glucose-6-phosphatase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Histochemical techniques were applied to whole mounts, to study the distribution of the enzymes alkaline phosphatase, acid phosphatase, adenosine triphosphatase, 5'-nucleotidase and glucose-6-phosphatase in the organs and tissues of a viviparous monogenean, Macrogyrodactylus clarii Gussev, 1961, from the gills of the North African catfish Clarias gariepinus (Burchell) in Egypt. The following organs and tissues were studied: head region, anterior adhesive glands, mouth region, pharynx, intestine, testis, vesicula seminalis, male accessory gland, male accessory reservoir, copulatory organ, receptaculum seminis, egg-cell forming region, embryonic cells, excretory system, nerve cells, haptor, muscle fibres and subtegumental cell bodies (cytons). The enzymes showed marked differences in their activities among the studied organs and tissues. Alkaline phosphatase and acid phosphatase activities were detected in many organs and tissues, while the activities of adenosine triphosphatase, 5'-nucleotidase and glucose-6-phosphatase were restricted to a few organs. Although no positive reaction for any enzyme was observed in the anterior adhesive gland cells, a positive reaction for acid phosphatase was detected in the anterior adhesive areas. All enzymes showed marked activity in the digestive and excretory systems. The distribution of the enzymes in the tissues and organs of M. clarii is compared with those of other monogeneans, including other gyrodactylids parasitizing the same host fish. Some possible functions of the enzymes are discussed.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

43-52

Source:

Folia parasitologica | 2012 Volume:59 | Number:1

Harvested from:

CDK

Metadata only:

false