Heat-shock-induced glucose transporter 4 in the slow-twitch muscle of rats

Title:

Heat-shock-induced glucose transporter 4 in the slow-twitch muscle of rats

Creator:

Wu, P.-F., Luo, S.-C., and Chang, L.-C.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3969e715-ce8b-408d-a241-618e1664cd1c
uuid:3969e715-ce8b-408d-a241-618e1664cd1c
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, lipoic acid, skeletal muscle groups, heat-shock, wound migration, heat shock protein 70 (HSP70), glucose transporter 4 (GLUT4), 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

In this study, lipoic acid and heat shock treatments were applied to C2C12 myotubes and Sprague-Dawley rats to investigate changes in the heat shock protein 70 (HSP70) and glucose transporter 4 (GLUT4) in 4 different skeletal muscle groups. The results of western blotting indicated that treatment of lipoic acid for 24 h, heat-shock and combined lipoic acid and heat-shock which all increased the level of HSP70 substantially in C2C12 myotubes. However, either lipoic acid or heat-shock did not increase the level of GLUT4 in C2C12 myotubes. In an in vitro migration assay, lipoic acid increased wound migration only when it was applied for 3 h. Moreover, our in vivo results revealed that lipoic acid did not increase HSP70 and GLUT4 in all 4 different skeletal muscles. Furthermore, heat-shock increased HSP70 in all 4 different muscle groups, and heat-shock treatment alone increased the GLUT4 in the soleus muscle only, suggesting that the GLUT4 increased by heat-shock was slow-twitch muscle specific. Collectively, our results indicated that heat-shock is critical factor that modulates GLUT4 and HSP70 in the skeletal muscle of rats., P.-F. Wu, S.-C. Luo, L.-C. Chang., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:4

Harvested from:

CDK

Metadata only:

false