The relation between changes in non-photochemical quenching, low temperature fluorescence emission, and membrane ultrastructure upon binding of polyionic compounds and fragments of light-harvesting complex 2

Title:

The relation between changes in non-photochemical quenching, low temperature fluorescence emission, and membrane ultrastructure upon binding of polyionic compounds and fragments of light-harvesting complex 2

Creator:

Štys, D., Šiffel, P., Hunalová, I., and Nebesářová, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:37f59dd8-aef5-4e75-af93-4329506fda1f
uuid:37f59dd8-aef5-4e75-af93-4329506fda1f
issn:0300-3604
doi:10.1023/A:1007172408839

Subject:

decyldiamine, ethylenediamine, peptides, membrane fragments, phosphorylation, and Pisum sativum

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Experiments were performed to distinguish some of the proposed mechanisms by which thylakoid membranes regulate the performance of photosynthetic apparatus in relation to non-photochemical quenching, qN. Aliphatic diamines were used as uncouplers of transmembrane H+ gradient as they can be transported across the membrane at the expense of hydrogen cations. Diamines did not induce changes in low-temperature fluorescence emission but induced different changes in membrane ultrastructure. Positively charged peptides did not affect membrane ultrastructure but blocked qN. In addition, they caused an increase of low temperature fluorescence emission between 710 and 720 nm. For control peptide, the maximal fluorescence increase was found at 715 nm. Fragments of light-harvesting complex 2 in their phosphorylated and non-phosphorylated form shifted the position of this increase. We believe that peptides bind to membrane surface and reduce the mobility of membrane components whose migration is needed for observation of qN. Phosphorylated and non-phosphophorylated LHC2 fragments bind to different binding sites for corresponding forms of the protein. and D. Štys ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

325-334

Source:

Photosynthetica | 1999 Volume:37 | Number:2

Harvested from:

CDK

Metadata only:

false