A universal method for the isolation of photochemically active broken chloroplasts from conifer needles and its possible application in photosynthetic studies

Title:

A universal method for the isolation of photochemically active broken chloroplasts from conifer needles and its possible application in photosynthetic studies

Creator:

Holá, D., Kočová, Marie, Rothová, Olga, Hlízová, E., Fridrichová, L., Lhotáková, Z., and Albrechtová, Jana

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:379915a0-8767-4b8b-b178-15b55763e0e2
uuid:379915a0-8767-4b8b-b178-15b55763e0e2
issn:0300-3604
doi:10.1007/s11099-012-0028-4

Subject:

botanika, botany, photosynthesis, photosystem I, photosystem II, 2, and 58

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

We have developed a simple and an effective method for the isolation of photochemically active broken chloroplasts from conifer needles that can be applied for a wide variety of conifer species with needle-like leaves. The utilisation of this method in photosynthetic studies offers a possibility to examine the efficiency of almost any component of thylakoid electron-transport chain and to disclose information about individual parts of primary photosynthetic processes that would be otherwise difficult to obtain. Various aspects influencing the outcome of this procedure, including the amount of needles necessary for sufficient yields, the possible length and the conditions of their storage, the best method for their disruption, the composition and pH of isolation and storage buffers, the centrifugation sequence, etc., are discussed., D. Holá ... [et al.]., and Obsahuje bibliografii

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

291-304

Source:

Photosynthetica | 2012 Volume:50 | Number:2

Harvested from:

CDK

Metadata only:

false