Comparison of new ELISA method with established SDS-PAGE method for determination of muscle myosin heavy chain isoforms

Title:

Comparison of new ELISA method with established SDS-PAGE method for determination of muscle myosin heavy chain isoforms

Creator:

Jan Říčný and Tomáš Soukup

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:36054bbe-39c4-4eb6-9ec7-14dde932b45c
uuid:36054bbe-39c4-4eb6-9ec7-14dde932b45c

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, muscle fiber types, myosin heavy chains, SDS-PAGE, immunoreactions-ELISA, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

We developed a new method for the quantitative determination of myosin heavy chain (MyHC) isoforms taking advantage of immunochemical differences and based on the ELISA principle. In the present paper we compare anal ysis of MyHC isoforms using the SDS-PAGE and the ELISA methods in the same samples of adult female inbred Lewis strain euthyroid, hyperthyroid and hypothyroid rats. In all thyroid states, the same composition and corresponding changes of MyHC isoforms were determined using both methodological approaches in the slow soleus and the fast extensor digitorum longus muscles. Our results showed that ELISA can be used for a “semi-quantitative” or “comparative” measurement of MyHC isoforms in multiple muscle samples, but that it is neither more exact no r faster compared to SDS-PAGE., Jan Říčný., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:6

Harvested from:

CDK

Metadata only:

false