Detection of Ca2+-binding proteins of photosystem 2 particies by electrophoretic migration combined with terbium fluorescence

Title:

Detection of Ca2+-binding proteins of photosystem 2 particies by electrophoretic migration combined with terbium fluorescence

Creator:

Zhang, Lixin and Liang, HouGuo

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:30b92511-bd3d-4015-a0e8-19cc67b5ad8e
uuid:30b92511-bd3d-4015-a0e8-19cc67b5ad8e
urnnbn:urn:nbn:cz:aba007-000fpl

Subject:

electrophoresis, ethyleneglycol-éííiP-aminoethyl ether) N,N,N',N'-tetraaeetic acid (EGTA), and Spinacea oleracea

Type:

model:internalpart and TEXT

Format:

print

Description:

The electrophoretic migration rates of several proteins of photosystem 2 particles ffom spinách were much higher iii gels containing 1 mM Ca^^ than in gels containing 1 mM ethyleneglycol-í)w(P-aminoethyl ether) N,N,N',N'-tetraacetic acid (EGTA). Incubation of gels with terbium (Tb^^) and the corresponding Tb3‘'^-fluorescence were ušed to identity the Ca^^-binding proteins on the basis of selective occupation of Ca2+-binding sites with Tb^+. The 47, 43 and particularly 33 kDa polypeptides were most probably involved in Ca2+-binding.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

621-624 and 143-146

Source:

Photosynthetica | 1995 Volume:31 | Number:4

Harvested from:

CDK

Metadata only:

false