T7 polymerase-driven transcription is downregulated in metacyclic promastigotes and amastigotes of Leishmania mexicana

Title:

T7 polymerase-driven transcription is downregulated in metacyclic promastigotes and amastigotes of Leishmania mexicana

Creator:

Aygul Ishemgulova, Kraeva, Natalya, Faktorová, Drahomíra, Podešvová, Lucie, Julius Lukeš, and Yurchenko, Vyacheslav

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:24b3256a-537d-497a-b091-92fa784f89b6
uuid:24b3256a-537d-497a-b091-92fa784f89b6
issn:1803-6465
doi:10.14411/fp.2016.016

Subject:

genová exprese, Leishmania, gene expression, untranslated regions, Tet-inducible system, Leishmania mexicana, 2, and 59

Type:

model:article and TEXT

Format:

print, počítač, and online zdroj

Description:

In our previous work we established a T7 polymerase-driven Tetracycline-inducible protein expression system in Leishmania mexicana (Biagi, 1953). We used this system to analyse gene expression profiles during development of L. mexicana in procyclic and metacyclic promastigotes and amastigotes. The transcription of the gene of interest and the T7 polymerase genes was significantly reduced upon cell differentiation. This regulation is not locus-specific. It depends on untranslated regions flanking open reading frames of the genes analysed. In this paper, we report that the previously established conventional inducible protein expression system may not be suitable for studies on differentiation of species of Leishmania Ross, 1903 and protein expression systems might have certain limitations., Aygul Ishemgulova, Natalya Kraeva, Drahomíra Faktorová, Lucie Podešvová, Julius Lukeš, Vyacheslav Yurchenko., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

1-4

Source:

Folia parasitologica | 2016 Volume:63 | Number:1

Harvested from:

CDK

Metadata only:

false