Role of light-harvesting complex 2 dissociation in protecting the photosystem 2 reaction centres against photodamage in soybean leaves and thylakoids

Title:

Role of light-harvesting complex 2 dissociation in protecting the photosystem 2 reaction centres against photodamage in soybean leaves and thylakoids

Creator:

Zhang, Hai-Bo and Xu, Da-Quan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1e26c67f-c5a8-480a-8003-216ec5e67f50
uuid:1e26c67f-c5a8-480a-8003-216ec5e67f50
issn:0300-3604
doi:10.1023/B:PHOT.0000015462.71601.d7

Subject:

chlorophyll fluorescence, D1 protein, 5'-p-fluorosulfonylbenzoyl adenosine, Glycine, protein phosphorylation, and PS2 reaction centre

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The protective role of light-harvesting complex 2 (LHC2) dissociation from photosystem 2 (PS2) complex was explored by the 5'-p-fluorosulfonylbenzoyl adenosine (FSBA, an inhibitor of protein kinase) treatment at saturating irradiance (SI) in soybean leaves and thylakoids. The dissociation of some LHC2s from PS2 complex occurred after SI treatment, but FSBA treatment inhibited the dissociation as demonstrated by analysis of sucrose density gradient centrifugation of thylakoid preparation and low-temperature (77 K) chlorophyll (Chl) fluorescence. A significant increase in F0 and decrease in Fv/Fm occurred after SI, and the two parameters could largely recover to the levels of dark-adapted leaves after subsequent 3 h in the dark, but they could not recover in the FSBA-treated leaves at SI. Neither the electron transport activity of PS2 nor the D1 protein amount in vivo had significant change after SI without FSBA, whereas FSBA treatment at SI could result in significant decreases in both the PS2 electron transport activity and the D1 protein amount. When thylakoids instead of leaves were used, the PS2 electron transport activity and the D1 protein amount declined more after SI with FSBA than without FSBA. The phosphorylation level of PS2 core proteins increased, while the phosphorylation level of LHC2 proteins was reduced after SI. Also, the phosphorylation of PS2 core proteins could be greatly inhibited by the FSBA treatment at SI. Hence in soybean leaf the LHC2 dissociation is an effective strategy protecting PS2 reaction centres against over-excitation and photodamage by reducing the amount of photons transferred to the centres under SI, and the phosphorylation of PS2 core proteins plays an important role in the dissociation. and Hai-Bo Zhang, Da-Quan Xu.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

383-391

Source:

Photosynthetica | 2003 Volume:41 | Number:3

Harvested from:

CDK

Metadata only:

false