Chronic low-dose L-NAME treatment increases nitric oxide production and vasorelaxation in normotensive rats

Title:

Chronic low-dose L-NAME treatment increases nitric oxide production and vasorelaxation in normotensive rats

Creator:

Iveta Bernátová, Kopincová, J., Angelika Púzserová, Pavol Janega, and Pavel Babál

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1ce6d042-1b5a-4aa2-bf5b-bc58fea33d64
uuid:1ce6d042-1b5a-4aa2-bf5b-bc58fea33d64
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, hypertenze, krevní tlak, acetylcholin, hypertension, blood pressure, acetylcholine, cardiac and vascular structure, negative feedback regulation, serotonin, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

NG-nitro-L-arginine methyl ester (L-NAME) is a non-specific nitric oxide (NO) synthase inhibitor, commonly used for the induction of NO-deficient hypertension. The aim of this study was to investigate the effect of chronic low-dose administration of L-NAME on NO production, vascular function and structure of the heart and selected arteries of rats. Adult male Wistar rats were treated with L-NAME in the dose of approximately 1.5 mg/kg/day in drinking water for 8 weeks. Basal blood pressure (BP) of rats (determined by tail-cuff) was 112±3 mm Hg. The low-dose administration of L-NAME significantly elevated BP measured on the third and sixth week of treatment vs. controls by approximately 9 % and 12 %, respectively. After this period, BP of L-NAME-treated rats returned to the control values. The relative left ventricular mass, heart fibrosis and collagen III/collagen I ratio were not affected by L-NAME. Similarly, there were no alterations in the cross-sectional area and wall thickness/diameter ratio of the aorta and the femoral artery of LNAME- treated rats. NO synthase activity (determined by conversion of [3H]-L-arginine to [3H]-L-citrulline) was not altered in the hypothalamus of L-NAME-treated rats. Interestingly, chronic low-dose L-NAME treatment significantly elevated NO synthase activity in the left ventricle and aorta, increased endothelium-dependent acetylcholine-induced vasorelaxation and reduced serotonin-induced vasoconstriction of the femoral artery. The data suggest that chronic lowdose L-NAME treatment can increase NO production and vasorelaxation in normotensive rats without negative structural changes in the cardiovascular system., I. Bernátová, J. Kopincová, A. Púzserová, P. Janega, P. Babál., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:Suppl 2

Harvested from:

CDK

Metadata only:

false