bmpkci is highly expressed in a resistant strain of silkworm (Lepidoptera: Bombycidae): implication of its role in resistance to BmDNV-Z

Title:

bmpkci is highly expressed in a resistant strain of silkworm (Lepidoptera: Bombycidae): implication of its role in resistance to BmDNV-Z

Creator:

Chen, Ke-Ping, Chen, Hui-Qing, Tang, Xu-Dong, Yao, Qin, Wang, Lin-Ling, and Han, Xu

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:19962661-fb80-4cd1-9f50-c4060b92d428
uuid:19962661-fb80-4cd1-9f50-c4060b92d428

Subject:

PKCI, Bombyx mori, virus-resistance, densonucleosis virus, fluorescent differential display, and quantitative real-time PCR

Type:

article, model:article, and TEXT

Description:

Using the fluorescent differential display technique, a special band named Bm541 was identified by screening for differentially expressed genes in the resistant silkworm strain Qiufeng, the susceptible strain Huaba35, and the near isogenic line BC6, which carries the resistant gene to Bombyx mori densonucleosis virus (BmDNV). After applying the 5'RACE technique with specially designed primers, a 1148 bp cDNA clone containing a 387 bp open reading frame (ORF) was obtained. This gene was registered in GenBank under the accession number AY860950. The deduced amino acid sequence showed a 73.1% identity to the protein kinase C inhibitor (PKCI) of Drosophila pseudoobscura. In the deduced sequence of BmPKCI, the histidine triad (HIT) motif, which is essential for PKCI function, and the α-helix region, which is conserved among the PKCI family, were present. The data from quantitative real-time PCR (QRT-PCR) suggest that the expression levels of bmpkci in BC6 and Qiufeng both with BmDNV-Z are significantly higher than those in Huaba35, which indicate that BmPKCI plays a role in resistance to BmDNV-Z.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2007 Volume:104 | Number:3

Harvested from:

CDK

Metadata only:

false