Long-term effect of elevated CO2 on spatial differentiation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity in Norway spruce canopy

Title:

Long-term effect of elevated CO2 on spatial differentiation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity in Norway spruce canopy

Creator:

Hrstka, M., Urban, O., and Marek, M. V.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:16dfaa94-68d3-427f-9bd7-77de4a78dcc2
uuid:16dfaa94-68d3-427f-9bd7-77de4a78dcc2
issn:0300-3604
doi:10.1007/PL00022057

Subject:

down-regulation, nitrogen, photosynthesis, Picea abies, sun and shade needles, and vertical profile

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Total in vitro activity of RuBPCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) enzyme was assayed spectrophotometrically by the continuous measurement of 3-phosphoglycerate-dependent NADH oxidation in a coupled enzyme system. RuBPCO activities were found in the ranges 1.01-2.76 and 1.23-3.10 µmol(CO2) m-2 s- 1 in current Norway spruce needles growing in ambient (AC) and elevated (EC) CO2 concentration, respectively. RuBPCO activity in AC needles from the upper layer (U) was 11-15 % higher compared to those from the middle (M) layer, and even 44-56 % higher compared to the lower (L) layer of spruce crown. Over the vegetation season, we observed a highly significant decrease of RuBPCO activity in the EC-U needles from 3.10 (July) to 1.60 (October) µmol(CO2) m-2 s-1 as a consequence of downward feedback regulation. Moreover, this down-regulation was not caused by a non-specific decrease in total leaf nitrogen content. and M. Hrstka, O. Urban, M. V. Marek.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

211-216

Source:

Photosynthetica | 2005 Volume:43 | Number:2

Harvested from:

CDK

Metadata only:

false