Expression of cytochrome P450 CYP6B6 in the different developmental stages of the insect Helicoverpa armigera (Lepidoptera: Noctuidae)

Title:

Expression of cytochrome P450 CYP6B6 in the different developmental stages of the insect Helicoverpa armigera (Lepidoptera: Noctuidae)

Creator:

Zhang, Xuetao, Yuan, Dan, Ding, Li, Li, Pan, Li, Fen, and Liu, Xiaoning

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:149f1c5b-6d51-4054-b0ad-58e72edfb8bc
uuid:149f1c5b-6d51-4054-b0ad-58e72edfb8bc

Subject:

Zoologie, zoologie, entomologie, Lepidoptera, Noctuidae, Helicoverpa armigera, developmental stage, CYP6B6 expression, 2, and 59

Type:

article, články, model:article, and TEXT

Description:

In order to determine when during the development of H. armigera CYP6B6 is expressed, we measured the width of the head capsule, the length of the body and weight of the different life stages of the cotton bollworm reared under laboratory condition. The larvae took about 20 d to complete their development under these conditions, the pre-pupae about 4 d, pupae about 9 d, and the adults lived for about 9 d and the eggs took about 4 d to hatch. Knowing this it was possible to determine the pattern of cytochrome P450 CYP6B6 expression at different stages in the life cycle of H. armigera using real-time quantitative polymerase chain reaction (RT-qPCR). The CYP6B6 gene was expressed in all the developmental stages of the cotton bollworm. The results indicate that the level of expression was lower in the egg, pre-pupal, pupal and adult stages than in larvae. The level of expression of CYP6B6 decreased from the 1st to the 3rd instar larva, with the lowest level recorded in the 3rd instar larvae and then increased reaching its highest value in 6th instar larvae. A similar trend in the expression of CYP6B6 was detected using immunohistochemistry. As the results show, more efficient control of the cotton bollworm can be achieved if insecticides are applied when the larvae of H. armiger are in the 3rd instar, because at that stage the eat little food and there is a low level of detoxifying enzymes in the larvae., Xuetao Zhang ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

European Journal of Entomology | 2013 Volume:110 | Number:1

Harvested from:

CDK

Metadata only:

false