Preparation of monoclonal antibodies against Bc48 and development of a rapid detection assay for infection with Babesia caballi in China

Title:

Preparation of monoclonal antibodies against Bc48 and development of a rapid detection assay for infection with Babesia caballi in China

Creator:

Wang, Panju, Song, Jingjing, Song, Ruiqi, Zhang, Mengyuan, Wu, Lijiang, Li, Fangxin, Yan, Yan, Zhou, Jiyong, Chahan, Bayin, and Liao, Min

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:114a7a3d-1c6c-4958-9ce5-b9be252979e3
uuid:114a7a3d-1c6c-4958-9ce5-b9be252979e3
issn:1803-6465
doi:10.14411/fp.2019.005

Subject:

infekce, infections, Čína, China, Rhoptry protein of B. caballi (Bc48), hybridoma cells, colloidal gold-immunochromatographic test, 2, and 59

Type:

model:article and TEXT

Format:

print, počítač, and online zdroj

Description:

Babesia caballi (Nuttal, 1910) is one of the causative agents of equine piroplasmosis which causes economic losses to horse industry in China. There is an urgent need for rapid detection method for B. caballi infection in Xinjiang Province, China. To prepare monoclonal antibodies (mAbs) against Bc48 gene of B. caballi (Xinjiang local strains) and establish colloidal gold-immunochromatographic (ICT) assay for diagnosis of the disease, recombinant Bc48 was expressed and purified from Escherichia coli. With purified Bc48 as immunogen in mice, three hybridoma cells named 11F4, 1H2 and 7F4 secreting mAbs against Bc48 of B. caballi were obtained, which showed strong reaction with recombinant Bc48 and Bc48 gene transfected cells. Furthermore, colloidal gold labelled ICT assay based on purified Bc48 recombinant antigen and its mAb was developed. The ICT assay showed high sensitivity and specificity and no cross-reaction with Theileria equi (Laveran, 1901). Total of 56 horse serum samples collected from Xinjiang were tested by ICT and compared with the detection by commercial ELISA kit. The results showed that 32 out of 56 serum samples were positive by ICT and 33 were positive by ELISA. ICT assay had high coincidence (98%) to the reference ELISA kit. mAbs and ICT developed in this study could be provided as an efficient diagnosis tool for infection with B. caballi in horse in Xinjiang area., Panju Wang, Jingjing Song, Ruiqi Song, Mengyuan Zhang, Lijiang Wu, Fangxin Li, Yan Yan, Jiyong Zhou, Bayin Chahan, Min Liao., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

1-6

Source:

Folia parasitologica | 2019 Volume:66 | Number:1

Harvested from:

CDK

Metadata only:

false