UV-B radiation mediated alterations in the nitrate assimilation pathway of crop plants. 2.. Kinetic characteristics of nitrite reductase

Title:

UV-B radiation mediated alterations in the nitrate assimilation pathway of crop plants. 2.. Kinetic characteristics of nitrite reductase

Creator:

Balakumar, T., Sathiameena, K., Selvakumar, V., Murugu Ilanchezhian, C., and Paliwal, K.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:064e3650-89f9-4141-8b22-4c9b235dceb3
uuid:064e3650-89f9-4141-8b22-4c9b235dceb3
issn:0300-3604
doi:10.1023/A:1007168029162

Subject:

nitrate reductase, nitrite reductase, enzyme induction and kinetics, and Vigna unguiculata

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Cowpea [Vigna unguiculata (L.) Walp. cv. Co 4] seedlings were subjected to a weighted irradiance of 3.2 W m-2 s-1 of biologically effective ultraviolet-B radiation (UV-B, 280-320 nm) and the changes in the kinetic and other characteristics of nitrite reductase (NiR) were recorded. The activity of NiR was hampered by 19 % under UV-B irradiation compared to the control. The UV-B treated plants required higher concentrations of nitrate for the induction of NiR synthesis than the controls. The NiR activity decay kinetics showed that the UV-B treatment significantly lowers the t1/2 of the enzyme, thereby indicating a reduced rate of enzyme turnover. The comparison of kinetic characteristics of nitrate reductase (NR) and NiR under UV-B treatment showed that NiR was not so sensitive to UV-B radiation as NR. As shown by enzyme turnover rates, NiR extracted from plants irradiated by UV-B in situ was less sensitive to UV-B radiation than the enzyme extract subjected to in vitro UV-B irradiation. Though NiR was less damaged by UV-B treatment than NR, subtle changes occurred in its kinetic characteristics. and T. Balakumar ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

469-475

Source:

Photosynthetica | 1999 Volume:37 | Number:3

Harvested from:

CDK

Metadata only:

false