N-(2-hydroxypropyl) methacrylamide based cryogels - synthesis and biomimetic modification for stem cell applications

Title:

N-(2-hydroxypropyl) methacrylamide based cryogels - synthesis and biomimetic modification for stem cell applications

Creator:

Golunova, A., Jaroš, J., Jurtíková, V., Kotelnikov, I., Kotek, J., Hlídková, H., Libor Streit, Aleš Hampl, František Rypáček, and Proks, V.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:0554a1fe-db29-4723-952e-83003d4ee783
uuid:0554a1fe-db29-4723-952e-83003d4ee783
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, hydrogely, kmenové buňky, popraviště, hydrogels, stem cells, scaffolds, cryogels, mechanical properties, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryoyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims., A. Golunova, J. Jaroš, V. Jurtíková, I. Kotelnikov, J. Kotek, H. Hlídková, L. Streit, A. Hampl, F. Rypáček, V. Proks., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false