ALKB-8, a 2-oxoglutarate-dependent dioxygenase and S-adenosine methionine-dependent methyltransferase modulates metabolic events linked to lysosome-related organelles and aging in C. elegans

Title:

ALKB-8, a 2-oxoglutarate-dependent dioxygenase and S-adenosine methionine-dependent methyltransferase modulates metabolic events linked to lysosome-related organelles and aging in C. elegans

Creator:

Kollárová, J., Kostrouchová, M., and Benda, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:03ba38d0-ee6c-4916-aec7-5fd2c4b967b6
uuid:03ba38d0-ee6c-4916-aec7-5fd2c4b967b6

Subject:

lysosome-related organelle, methyltransferase, multifunctional enzyme, Nile red, oxidoreductase, RNA-binding, and transferase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

ALKB-8 is a 2-oxoglutarate-dependent di-oxygenase homologous to bacterial AlkB, which oxidatively demethylates DNA substrates. The mammalian AlkB family contains AlkB homologues denominated ALKBH1 to 8 and FTO. The C. elegans genome includes five AlkB-related genes, homologues of ALKBH1, 4, 6, 7, and 8, but lacks homologues of ALKBH2, 3, and 5 and FTO. ALKBH8 orthologues differ from other AlkB family members by possessing an additional methyltransferase module and an RNA binding N-terminal module. The ALKBH8 methyltransferase domain generates the wobble nucleoside 5-methoxycarbonylmethyluridine from its precursor 5-carboxymethyluridine and its (R)- and (S)-5-methoxycarbonylhydroxymethyluridine hydroxylated forms in tRNAArgUCG and tRNAGlyUCC. The ALKBH8/ALKB-8 methyltransferase domain is highly similar to yeast TRM9, which selectively modulates translation of mRNAs enriched with AGA and GAA codons under both normal and stress conditions. In this report, we studied the role of alkb-8 in C. elegans. We show that downregulation of alkb-8increases detection of lysosome-related organelles visualized by Nile red in vivo. Reversely, forced expression of alkb-8 strongly decreases the detection of this compartment. In addition, overexpression of alkb-8 applied in a pulse during the L1 larval stage increases the C. elegans lifespan. and Corresponding author: Marta Kostrouchová

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

[46]-58

Source:

Folia biologica | 2018 Volume:64 | Number:2

Harvested from:

CDK

Metadata only:

false