Characterization and transcriptional analysis of a subtelomeric satellite DNA family in the ladybird beetle Henosepilachna argus (Coleoptera: Coccinellidae)

Title:

Characterization and transcriptional analysis of a subtelomeric satellite DNA family in the ladybird beetle Henosepilachna argus (Coleoptera: Coccinellidae)

Creator:

Mora, Pablo, Vela, Jesús, Palomeque, Teresa, Ruiz-Mena, Areli, and Lorite, Pedro

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:0387a3d0-720e-4b1b-b842-25ba45d3b638
uuid:0387a3d0-720e-4b1b-b842-25ba45d3b638
issn:1210-5759
doi:10.14411/eje.2017.061

Subject:

Zoologie, brouci, slunéčkovití, transkripce, beetles, Coccinellidae, transcription, Coleoptera, Henosepilachna argus, ladybird beetle, satellite DNA, in situ hybridization, 2, and 59

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, počítač, and online zdroj

Description:

Satellite DNAs are the major repetitive DNA components in eukaryotic genomes. Although satellite DNA has long been called "parasite DNA" there is substantial evidence that it could be associated with some functions of chromosome biology. Ladybird beetles (Coccinellidae) are one of the largest and most important groups of beetles. Many ladybirds are of economic interest as biological control agents because they eat some agricultural pests such as aphids and scale insects. However, other species are phytophagous and can damage crops. Despite the ecological importance of the latter group there are no studies on their satellite DNA. A satellite DNA family was isolated and characterized in the ladybird Henosepilachna argus. This satellite DNA is organized in tandem repeats of 658 bp and is A + T rich (67.3%). The recorded high sequence conservation of the monomers together with the detection of putative gene conversion processes indicate concerted evolution. Reverse transcription polymerase chain reaction (RT-PCR) revealed that this satellite DNA is transcribed and in situ hybridization its location in the subtelomeric regions of all chromosomes except the long arm of the X chromosome. The presence of this satellite DNA in other species of the genus Henosepilachna and Epilachna was also tested using PCR. The results indicate that this satellite DNA sequence is so far specific to H. argus., Pablo Mora, Jesús Vela, Areli Ruiz-Mena, Teresa Palomeque, Pedro Lorite., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

European Journal of Entomology | 2017 Volume:114

Harvested from:

CDK

Metadata only:

false