Effects of low-frequency electromagnetic fields (LF EMF) on the
activation of different tissue recovery processes have not yet
been fully understood. The detailed quantification of LF EMF
effects on the angiogenesis were analysed in our experiments by
using cultured human and mouse endothelial cells. Two types of
fields were used in the tests as follows: the LF EMF with
rectangular pulses, 340-microsecond mode at a frequency of
72 Hz and peak intensity 4 mT, and the LF EMF with sinusoidal
alternating waveform 5 000 Hz, amplitude-modulated by means
of a special interference spectrum mode set to a frequency linear
sweep from 1 to 100 Hz for 6 s and from 100 Hz to 1 Hz return
also for 6 s, swing period of 12 second. Basic parameters of
cultured cells measured after the LF EMF stimulus were viability
and proliferation acceleration. Both types of endothelial cells
(mouse and human ones) displayed significant changes in the
proliferation after the application of the LF EMF under conditions
of a rectangular pulse mode. Based on the results, another test
of the stimulation on a more complex endothelial-fibroblast
coculture model will be the future step of the investigation.