Sorbus eximia Kovanda, a hybridogenous species that originated from the parental combination S. torminalis and S. aria s.l., is thought to be an apomictic species, which includes diploid and tetraploid individuals. The present study confirmed the existence of only triploid individuals. A new tentatively apomictic triploid (2n = 3x = 51) species from the S. latifolia group: S. barrandienica P. Vít, M. Lepší et P. Lepší is described based on a revision of S. eximia. This species is assumed to have originated from a cross between S. danubialis or S. aria s.l. and S. torminalis. A wide palette of biosystematic techniques, including molecular (nuclear microsatellite markers) and karyological analyses (chromosome counts, DAPI flow cytometry) as well as multivariate morphometric and elliptic Fourier analyses, were used to assess the variation in this species and justify its independent taxonomic status. Allopatric occurrences of both species were recorded east of the town of Beroun in the Český kras, central Bohemia (Bohemian Karst). A distribution map of the two species is provided. Sorbus eximia occurs at four localities (the total number of adults and juveniles is 100 and 200, respectively) in basiphilous thermophilous oak forests (Quercion pubescenti-petraeae), mesic oak forests (Melampyro nemorosi-Carpinetum), woody margins of dry grasslands (Festucion valesiacae) and pine plantations. Sorbus barrandienica has so far been recorded at 10 localities (ca 50 adults). Recent field studies failed to verify two of these localities. It is mainly found growing on the summits of hills, usually in thermophilous open forests (Primulo veris-Carpinetum, Melampyro nemorosi-Carpinetum, Quercion pubescenti-petraeae) and woody margins of dry grassland. Its populations exhibit minimal genetic variation and are phenotypically homogeneous and well separated from other Bohemian hybridogenous Sorbus species. The epitype of S. eximia is designated here, and a photograph of the specimen is included. Photographs of the type specimens and in situ individuals, and line drawings of both species are presented.