This study introduces the concept of political disaffection, its measurement and operationalisation. Theoretically, this article builds on a differentiation between four basic types of orientations towards a political regime and its institutions: legitimacy of the regime, institutional disaffection, individual disaffection, and political dissatisfaction. Political disaffection is composed of two dimensions: institutional disaffection refers to beliefs that political institutions are not responsive to the requirements of the people; and individual disaffection reflects citizens‘ perceptions that they are able and willing to participation in politics. Principal axis factoring, reliability analysis along with internal and external validity analysis are used to examine institutional and individual disaffection using the Czech waves within ISSP (1996, 2000, 2004 and 2006). The results indicate that items used for measuring institutional and individual disaffection do measure the two concepts of interest. Moreover, repeated measurement of political disaffection and the stability of the results obtained provide strong arguments for the usage of these measures in future surveys.