Renal activity of Akt kinase in experimental type 1 diabetes
- Title:
- Renal activity of Akt kinase in experimental type 1 diabetes
- Creator:
- Jana Ždychová, Jana Veselá, Ludmila Kazdová, and Radko Komers
- Identifier:
- https://cdk.lib.cas.cz/client/handle/uuid:e51fc3c2-8edf-439b-ad96-68e4f3a31dd5
uuid:e51fc3c2-8edf-439b-ad96-68e4f3a31dd5
issn:0862-8408 - Subject:
- Fyziologie člověka a srovnávací fyziologie, diabetologie, kinázy, diabetická nefropatie, inzulín, diabetology, kinases, diabetic nephropathy, insulin, Akt kinase, mammalian target of rapamycin (mTOR), 14, and 612
- Type:
- article, články, model:article, and TEXT
- Format:
- print, bez média, and svazek
- Description:
- Akt kinase regulates numerous cell functions including glucose metabolism, cell growth, survival, protein synthesis, and control of local hemodynamics. mTOR is one of down-stream effectors of Akt involved in the initiation of protein translation. However, renal Akt signaling in Type 1 diabetes (DM) in vivo, in particular under the conditions reflecting differences in metabolic control, has received less attention. Renal cortical activity and expression of Akt and mTOR (kinase assay, western blotting) were determined in streptozotocin-diabetic rats (D) with different levels of glycemic control (blood glucose 22.0± 1.0, 13.4±1.5, 8.1±0.4 mmol/l, p<0.05 between the groups), achieved by varying insulin treatment (0,4 and 12 IU/day), and in control rats with (C4) or without (C) chronic insulin administration. Renal Akt activity was reduced in D rats without insulin treatment and severe hyperglycemia (D-0, -62 %, p<0.01 vs. C), partially restored in moderately hypergly cemic rats (D-4, -30 %, p<0.05 vs. C), and normalized in D rats with intensive insulin and tight metabolic control (D-12). Expression of active mTOR paralleled Akt activity in D-0 (-51 %, p<0.01 vs. C), but not in D-4 and D- 12 that demonstrated increases in active mTOR (+55 %, +80 % resp., p<0.05) as compared to C. Moreover, insulin activated renal Akt (+82 %, p<0.01), but not mTOR in C4. In conclusion, glycemic control and intensity of insulin treatment are important modulators of renal Akt and mTOR activity in diabetes. While Akt activity is reversible by tight metabolic control, combination of hyperglycemia and insulin treatment resulted in enhancement of mTOR activity. In addition to Akt, other signaling pathways likely contribute to regulation of renal mTOR activity in diabetes., J. Ždychová, J. Veselá, L. Kazdová, R. Komers., and Obsahuje bibliografii a bibliografické odkazy
- Language:
- English
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public - Source:
- Physiological research | 2008 Volume:57 | Number:5
- Harvested from:
- CDK
- Metadata only:
- false
The item or associated files might be "in copyright"; review the provided rights metadata:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- policy:public