Increase of the expression and activity of ferredoxin-NADP+ oxidoreductase in the cells adapted to low CO2 in the cyanobacterium Synechocystis 6803

Title:

Increase of the expression and activity of ferredoxin-NADP+ oxidoreductase in the cells adapted to low CO2 in the cyanobacterium Synechocystis 6803

Creator:

Liu, Y. R., Ma, W. M., and Mi, H. L.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:90364df9-cd67-4d46-bc35-c9206c1f6db5
uuid:90364df9-cd67-4d46-bc35-c9206c1f6db5
issn:0300-3604
doi:10.1007/s11099-010-0054-z

Subject:

botanika, botany, expression and activity, ferredoxin-NADP oxidoreductase, low CO2, and Synechocystis 6803

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

To investigate the effect of low CO2 on the expression and activity of ferredoxin-NADP+ oxidoreductase (FNR) and this enzyme-mediated cyclic electron flow around photosystem I (cyclic PSI), the activity staining, immunoblotting and initial rate of P700 + reduction were measured in high- or low-CO2-grown (H or L)-cells of wild-type Synechocystis sp. strain PCC 6803 (WT) and its ΔndhB mutant (M55). Major results were depicted as follows. (1) The protein levels and activity of FNR were remarkably stimulated in L-cells of both WT and M55 relative to that in their H-cells. (2) The rate of cyclic PSI was significantly increased in L-cells of WT, not M55, when compared to that in respective H-cells. (3) N-ethylmaleimide, an inhibitor of FNR, partially inhibited the increase in the rate of cyclic PSI induced by low CO2 in both WT and M55. These findings indicated that low CO2 enhanced the expression and activity of FNR and the cyclic PSI mediated by FNR. The contribution of FNR to cyclic PSI is shortly discussed. and Y. R. Liu, W. M. Ma, H. L. Mi.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

417-420

Source:

Photosynthetica | 2010 Volume:48 | Number:3

Harvested from:

CDK

Metadata only:

false