Effect of long-term NO synthase inhibition on cyclic nucleotide content in rat tissues

Title:

Effect of long-term NO synthase inhibition on cyclic nucleotide content in rat tissues

Creator:

Pecháňová, O. and Bernátová, I.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:8f831565-c800-4906-bfca-864f16cbfcf5
uuid:8f831565-c800-4906-bfca-864f16cbfcf5

Subject:

NO synthase, L-NAME, cGMP, cAMP, and hypertrophy

Type:

article, model:article, and TEXT

Description:

The effect of 4 weeks’ inhibition of NO synthase by nitro-L-arginine methyl ester (L-NAME) on haemodynamic parameters and cGMP and cAMP content was studied in rat tissues. L-NAME in both 20 mg/kg/day and 40 mg/kg/day doses significantly increased systolic blood pressure by 28 % and 30 % and decreased the heart rate by 14 % and 23 %, respectively, after the first week. These changes persisted during the following three weeks. Left ventricular weight/body weight (LVW/BW) ratio was significantly elevated in both L-NAME-treated groups by 19 % and 29 %, respectively. Radioimmunoassay was used to determine the cGMP and cAMP content Cyclic GMP content in animals treated by L-NAME (20 mg/kg/day and 40 mg/kg/day) decreased significantly by 13 % and 22 % in the left ventricle, by 28 % and 62 % in the aorta, by 20 % and 34 % in the brain, and by 10 % and 15 % in the kidney, respectively. On the other hand, the cAMP content increased in both L-NAME treated groups by 8 % and 9 % in the left ventricle, by 28 % and 46 % in the aorta, and by 23 % and 32 % in the brain, respectively. There were no significant changes in kidney cAMP content as compared to control animals. The results suggest a simultaneous decrease of cGMP and increase of cAMP content in the majority of studied tissues during NO-deficient hypertension.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1996 Volume:45 | Number:4

Harvested from:

CDK

Metadata only:

false