TSG-6 induces apoptosis of human hypertrophic scar fibroblasts via activation of theFas/FasL signalling pathway

Title:

TSG-6 induces apoptosis of human hypertrophic scar fibroblasts via activation of theFas/FasL signalling pathway

Creator:

Li, X.-Y., Li, T., Li, X.-J., Wang, J.-N., and Chen, Z.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:6a8785af-cfc7-4d73-91ee-068feb736993
uuid:6a8785af-cfc7-4d73-91ee-068feb736993

Subject:

TSG-6, hypertrophic scar fibroblast, apoptosis, and Fas/FasL

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Tumour necrosis factor-stimulated gene 6 (TSG6) is a protective inflammatory reaction gene which is upregulated by inflammatory processes. Recent studies suggest that TSG-6 exhibits anti-scar-ring effects. However, the mechanism of TSG-6 action in the scar formation remains poorly understood. We investigated whether TSG-6 affects growth of the human hypertrophic scar fibroblasts (HSFs) via Fas/FasL signalling pathway. Cultured HSFs were transfected with a vector carrying the TSG6gene (pLVX-Puro-TSG-6) or with a vector not containing the TSG6 gene (pLVX-Puro). Untransfected HSFs served as a control group to both transfected HSFs. The expressions level of TSG-6 was up-regulated in the pLVX-Puro-TSG-6 group at the protein and mRNA level. MTT and flow cytometry were used to assess the effect of TSG-6 on the growth and apoptotic status of HSFs. Finally, qRT-PCR and western blot were used to measure the expression levels of Fas, FasL, FADD, caspase-3 and caspase-8 in each group. The apoptosis rate was significantly enhanced and the growth rate reduced in the HSFs transfected with the TSG6 gene vector. The expression levels of Fas, FasL, FADD, caspase-3 and caspase-8 were significantly raised in the TSG-6 overexpressing HSFs. It is concluded that increased expression of TSG-6 may induce apoptosis of human hypertrophic scar fibroblasts via activation of the Fas/FasL signalling pathway. and Corresponding author: Xiao-Jing Li

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

[173]-181

Source:

Folia biologica | 2018 Volume:64 | Number:5-6

Harvested from:

CDK

Metadata only:

false