Macrophages are novel sites of expression and regulation of retinol binding protein-4 (RBP4)

Title:

Macrophages are novel sites of expression and regulation of retinol binding protein-4 (RBP4)

Creator:

Broch, M., Ramírez, R., Auguet, M. T., Alcaide, M. J., Aguilar, C., Garcia-España, A., and Richart, C.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:3f7292a6-fffb-4eac-ba72-5f82fb2df731
uuid:3f7292a6-fffb-4eac-ba72-5f82fb2df731

Subject:

Fyziologie člověka a srovnávací fyziologie, obezita, obesity, RBP4, primary human macrophages, inflammatory markers, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Obesity is linked to a low-level chronic inflammatory state that may contribute to the development of associated metabolic complications. Retinol-binding protein 4 (RBP4) is an adipokine associated with parameters of obesity including insulin resistance indices, body mass index, waist circumference, lipid profile, and recently, with circulating inflammatory factors. Due to the infiltration of adipose tissue in obesity by macrophages derived from circulating monocytes and, on the other hand, the existence of a close genetic relationship between adipocytes and macrophages, we decided to examine if RBP4 is expressed in monocytes and/or primary human macrophages. While we did not detect expression of RBP4 in undifferentiated monocytes, RBP4 expression became evident during the differentiation of monocytes into macrophages and was highest in differentiated macrophages. Once we demonstrated the expression of RBP4 in macrophages, we checked if RBP4 expression could be regulated by inflammatory stimuli such as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), or the endotoxin lipopolysaccharide (LPS). We observed that while RBP4 expression was strongly inhibited by TNF-α and LPS, it was not affected by IL-6. Our results highlight the complexity behind the regulation of this adipokine and demonstrate that RBP4 expression in macrophages could be modulated by inflammatory stimuli., M. Broch ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:2

Harvested from:

CDK

Metadata only:

false