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Start Over Subject radiologie Date Unknown

2. Expression of phospho-Elk-1 in rat gut after the whole body gamma irradiation

Creator:
Daniel Driák, Jan Österreicher, Zuzana Řeháková, Zdena Vilasová, and Jaroslava Vávrová
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Experimentální medicína, radiologie, biologické markery, radiology, biological markers, radiation-induced entero-colitis, phospho-Elk-1, biodosimetric marker, 14, and 616-092
Language:
English
Description:
Gastrointestinal form is the second stage of acute radiation syndrome (ARS) with a threshold dose of 8 Gy in man. It represents an absolutely lethal clinical-pathological unit, necro-hemorrhagic enteritis and proctocolitis, with unknown causal therapy. Elk-1 is a protein acting as a transcription factor activating specified genes. The purpose of our study was to examine the expression of phospho-Elk-1 in irradiated jejunum and transversal colon of rats with radiation-induced enterocolitis and to assess the importance of this transcriptional factor as a biodosimetric marker of radiation-induced enteropathy. The laboratory rats were randomly divided into 21 groups, 10 animals per group, and irradiated with whole body γ-irradiation of 1, 5, 10, 15, and 20 Gy. Samples of jejunum and transversal colon were taken 24, 48, 72, and 96 hours later, immunohisto-chemically stained, and the phospho-Elk-1 expression was examined using computer image analysis. A group of 10 sham-irradiated animals was used as control. Significantly increased expression of phospho-Elk-1 in rat jejunum has been found in all time intervals after irradiation by sublethal doses of 1 and 5 Gy, whereas after the irradiation by lethal doses, the expression of phospho-Elk-1 in rat jejunum varied considerably. Significantly increased expression of phospho-Elk-1 in transversal colon has also been found in the first days after irradiation by sublethal doses of 1 and 5 Gy. After irradiation by lethal doses, tere was no uniform pattern of the changes in the expression of phospho-Elk-1 in rat transversal colon. The detection of phospho-Elk-1 might be considered as a suitable and very sensitive biodosimetric marker of radiation-induced injury of small and large intestine. According to our knowledge, this is the first study on the phospho-Elk-1 expression in irradiated jejunum and transversal colon in the rat., D. Driák, J. Österreicher, Z. Řeháková, Z. Vilasová, J. Vávrová., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Radiologická fyzika a fyzika v medicíně

Creator:
Solný, Pavel
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
lékařská fyzika, radiologie, radiologická fyzika, medical physics, radiology, radiological physics, 6, and 53
Language:
Czech
Description:
Implementation of new technologies in medicine is historically connected via a collaboration between physicians and physicists. Through many of the new medicine methods were famously in the spotlight, the technology and physics remained hidden in the background. Interestingly. it is true that at the outset many of these new technologies were implemented into medical practice without any proper study of its physical basis, risk assessment or appropriate research (an example of this is radiotherapeutics use of X-rays within 6 months after their discovery). Current state-of-the-art of medicine requires a close cooperation between physicians and clinician technologists, and in the case of the use of ionizing radiation, also medical physicists. Correct cooperation is the basis of up-to-date medical care, which now more than ever before, is a multidisciplinary branch., Pavel Solný., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public