Four laboratory-hatched European kestrels Falco tinnunculus L. were fed on laboratory mice and common voles Microtus arvalis Pallas previously inoculated with different doses of sporulated oocysts of Caryospora kutzeri Böer, 1982. Two kestrels that were fed infected mice shed C. kutzeri oocysts 6 days after ingesting murine tissues. To compare direct and indirect transmissions, two of the kestrels were subsequently directly inoculated with 105 sporulated C. kutzeri oocysts and became patent on days 8 and 9 and shed caryosporan oocysts up to day 25 post inoculation. Additionally, four mice were inoculated with 106 oocysts in order to examine mouse tissues for the presence of developmental stages of C. kutzeri. No coccidian stages were found in the tissues of inoculated mice. The experiment showed that developmental stages of C. kutzeri are able to survive in mouse tissues and cause infection of suitable host after their ingestion.
Aim of this work is the optical design of high resolution imaging spectrograph which is used for Raman’s spectroscopy. It works in a range of wavelength 205 nm - 273 nm. The design is realized as a combination of mirrors and lenses system. There is a diffraction grating with dispersive prism between these systems. One mirror and one lens have an aspheric surface for minimizing final aberrations. Point spread function (PSF) of design for criterium 1/e2 is smaller than 27 μm for full FOV (field of view) and the main wavelengths. and Cílem této práce je představit návrh optického zobrazovacího spektrografu s vysokým rozlišením, který je určen pro Ramanovu spektroskopii. Spektrograf pracuje ve spektrálním rozsahu 205 nm až 273 nm. V návrhu je použita kombinace zrcadlového a čočkového optického systému, mezi nimiž je umístěna odrazná difrakční mřížka s dvěma disperzními hranoly. Pro minimalizování optických aberací má jedno zrcadlo a jedna čočka asférický povrch. Bodová rozptylová funkce (PSF) pro kritérium 1/e2 (plná šířka v 1/e2 = 0,135 násobku maxima) je pro celé zorné pole a hlavní vlnové délky menší než 27 μm.